Part:BBa_K187021:Design
Kanamycin resistance gene in pBA, BioBytes plasmid
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 10
Illegal PstI site found at 193
Illegal PstI site found at 811 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 193
Illegal PstI site found at 811 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 10
Illegal PstI site found at 193
Illegal PstI site found at 811 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 10
Illegal PstI site found at 193
Illegal PstI site found at 811
Illegal NgoMIV site found at 644 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 493
Illegal SapI.rc site found at 703
Design Notes
This plasmid includes only the open reading frame (start codon to stop codon) of the kan resistance gene. A promoter and terminator are not included. pAB and pBA do include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express kan resistance, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto the kan resistance gene. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.
Source
The kanamycin resistance gene was cloned out of the plasmid pKD4. Biobytes plasmid pBA is entered as BBa_K187001.